The National Diagnostic Protocol 52 (NDP 52) for Maize dwarf mosaic virus (species: Potyvirus zeananus) (MDMV) was endorsed by the Subcommittee on Plant Health Diagnostics (SPHD) for use in the event of an incursion, providing an essential tool to protect Australia’s maize and sorghum industries.
Transmitted by a broad range of aphid species, MDMV causes mosaic or mottle symptoms in hosts. Severe infections can result in stunted growth, increased tillering, and poor seed set. The field host range of MDMV includes maize (Zea mays), Johnsongrass (Sorghum halepense), and Sudan grass (Sorghum sudanense), though the potential host range is likely much broader based on tested experimental hosts.
MDMV is not currently known to occur in Australia. Therefore, the endorsed NDP equips diagnosticians, biosecurity personnel, and industry representatives with a standardised and reliable method to diagnose and detect MDMV. The NDP also distinguishes MDMV from other closely related viruses that are common and widespread in Australia. This protocol plays a crucial role in the detection and effective management of this pest, and is a vital tool to safeguard and maintain market access for Australian maize and sorghum industries.
For more information on NDP 52 or on NDP development, review, and verification, or other NDP-related queries, please contact NDPcoordinator@phau.com.au.
We acknowledge the authors and reviewers for their contributions towards developing reviewing, and verifying this NDP. The progression and endorsement of this NDP were made possible with coordination and support from the Diagnostic Protocols Working Group (DPWG) and the Subcommittee on Plant Health Diagnostics (SPHD). The coordination of NDP progression was facilitated through funding support by the Australian Government Department of Agriculture, Fisheries and Forestry (DAFF) under the National Plant Biosecurity Diagnostics Professional Development and Protocols Project managed by Plant Health Australia (PHA).
Image credit: Craig Grau, Bugwood.org