Introduction to Illumina DNA library preparation
Next-generation sequencing (NGS) has become an essential tool across genetics, genomics, ecology, microbiology, agriculture, and biomedical research. DNA library preparation is often outsourced, leaving many students and researchers with little understanding of the process or the confidence to perform it themselves. In reality, library preparation is more accessible than many people expect, and developing this skill can significantly reduce project costs while providing greater flexibility for experimental design and troubleshooting.
This free in-person workshop from the ANU Centre for Biodiversity Analysis will introduce participants to the principles of Illumina DNA library preparation for short-read sequencing before guiding them through the complete laboratory workflow. Participants will gain practical experience with the key steps involved in library construction, quality control, and best laboratory practices, while also learning how library preparation influences sequencing performance and downstream analyses.
The workshop is designed for students and early career researchers with basic molecular biology laboratory experience who want to build practical genomics skills that can be immediately applied to their own research projects. If required, and based on registration responses, we will also offer an optional introductory laboratory session the day before the workshop to cover essential molecular biology techniques.