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A rapid and sensitive RPA‑Cas12a assay for detection of banana bunchy top virus
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This article, published in Tropical Plant Pathology, presents the first reported CRISPR-based diagnostic for BBTV, and the RPA-Cas12a assay described here provides a rapid, sensitive and specific method for BBTV detection. It demonstrates improved analytical sensitivity relative to existing commercial RPA kits, performs effectively on both dried and fresh banana leaf material and offers operational flexibility through both one-tube and two step workflows. These characteristics make it a strong candidate for integration into BBTV management programmes, particularly where rapid, low-resource testing is needed.
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